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Int J Med Sci. 2013 Aug 7;10(10):1286-94. doi: 10.7150/ijms.5985. eCollection 2013.

Microcurrent electrical nerve stimulation facilitates regrowth of
mouse soleus muscle.

Ohno Y1, Fujiya H, Goto A, Nakamura A, Nishiura Y, Sugiura T, Ohira Y, Yoshioka T, Goto K.
Author information
Abstract
Microcurrent electrical nerve stimulation (MENS) has been used to facilitate recovery from skeletal
muscle injury. However, the effects of MENS on unloading-associated atrophied skeletal muscle remain
unclear. Effects of MENS on the regrowing process of unloading-associated atrophied skeletal muscle
were investigated. Male C57BL/6J mice (10-week old) were randomly assigned to untreated normal
recovery (C) and MENS-treated (M) groups. Mice of both groups are subjected to continuous hindlimb
suspension (HS) for 2 weeks followed by 7 days of ambulation recovery. Mice in M group were treated
with MENS for 60 min 1, 3, and 5 days following HS, respectively, under anesthesia. The intensity, the
frequency, and the pulse width of MENS were set at 10 μA, 0.3 Hz, and 250 msec, respectively. Soleus
muscles were dissected before and immediately after, 1, 3 and 7 days after HS. Soleus muscle wet
weight and protein content were decreased by HS. The regrowth of atrophied soleus muscle in M group
was faster than that in C group. Decrease in the reloading-induced necrosis of atrophied soleus was
facilitated by MENS. Significant increases in phosphorylated levels of p70 S6 kinase and protein kinase B
(Akt) in M group were observed, compared with C group. These observations are consistent with that
MENS facilitated regrowth of atrophied soleus muscle. MENS may be a potential extracellular stimulus to
activate the intracellular signals involved in protein synthesis.
KEYWORDS:
atrophy, intracellular signal, microcurrent electrical nerve stimulation, regrowth, rehabilitation.,
skeletal muscle

PMID:

23983587

[PubMed - indexed for MEDLINE]

PMCID:

PMC3752717

Free PMC Article

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